RTN4 Antibody / Reticulon 4 / Nogo A

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NSJ Bioreagents
Product Code: NSJ-RQ5920
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5920-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Nogo A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE human prostate cancer with Nogo A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
2 / 5
IHC staining of FFPE human prostate cancer with Nogo A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Immunofluorescent staining of FFPE human U-2 OS cells with Nogo A antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 5
Western blot testing of human 1) U-87 MG and 2) SH-SY5Y lysate with Nogo A antibody. Predicted molecular weight ~130 kDa but may be observed at up to ~220 kDa.
5 / 5
Flow cytometry testing of human SiHa cells with Nogo A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nogo A antibody.
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Immunofluorescent staining of FFPE human prostate cancer with Nogo A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
IHC staining of FFPE human prostate cancer with Nogo A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with Nogo A antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) U-87 MG and 2) SH-SY5Y lysate with Nogo A antibody. Predicted molecular weight ~130 kDa but may be observed at up to ~220 kDa.
Flow cytometry testing of human SiHa cells with Nogo A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nogo A antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Nogo A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Human neurite outgrowth inhibitor (NOGO) cDNAs encodes 3 splice variants: NOGOA, NOGOB and NOGOC. The longest cDNA, designated NOGOA, has an open reading frame of 1192 amino acids. It is a potent inhibitor of neurite growth and an IN-1 antigen produced by oligodendrocytes, and may allow the generation of new reagents to enhance CNS regeneration and plasticity. Nogo-A, a member of the Reticulon family, is expressed by oligodendrocytes and associates primarily with the endoplasmic reticulum. The acidic amino terminus of Nogo-A is detected at the cytosolic face of cellular membranes and may contribute to inhibition of axon regeneration at sites of oligodendrocyte injury. A multivalent form of the N terminus of Nogo-A affects the morphology of both neurons and other cell types.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids VSNNILHNQQELPTALTKLV from the human protein were used as the immunogen for the Nogo A antibody.
Limitation:
This Nogo A antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9NQC3