DCN Antibody / Decorin

NSJ Bioreagents
Product Code: NSJ-F54863
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54863-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54863-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the DCN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Flow cytometry testing of human HepG2 cells with DCN antibody; Blue=isotype control, Green= DCN antibody.
2 / 4
Western blot testing of mouse heart tissue lysate with DCN antibody. Predicted molecular weight ~40 kDa.
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with DCN antibody.
4 / 4
IHC testing of FFPE human hepatocellular carcinoma tissue with DCN antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry testing of human HepG2 cells with DCN antibody; Blue=isotype control, Green= DCN antibody.
Western blot testing of mouse heart tissue lysate with DCN antibody. Predicted molecular weight ~40 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with DCN antibody.
IHC testing of FFPE human hepatocellular carcinoma tissue with DCN antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the DCN antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
DCN is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 122-150 from the human protein was used as the immunogen for the DCN antibody.
Limitation:
This DCN antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P07585