Hemoglobin subunit gamma-2 Antibody / HBG2

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NSJ Bioreagents
Product Code: NSJ-F54741
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54741-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54741-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Hemoglobin subunit gamma-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human liver tissue with Hemoglobin subunit gamma-2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE mouse liver tissue with Hemoglobin subunit gamma-2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human brain tissue lysate with Hemoglobin subunit gamma-2 antibody. Predicted molecular weight ~16 kDa.
4 / 4
Flow cytometry testing of human U-87 MG cells with Hemoglobin subunit gamma-2 antibody; Blue=isotype control, Green= Hemoglobin subunit gamma-2 antibody.
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IHC testing of FFPE human liver tissue with Hemoglobin subunit gamma-2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse liver tissue with Hemoglobin subunit gamma-2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human brain tissue lysate with Hemoglobin subunit gamma-2 antibody. Predicted molecular weight ~16 kDa.
Flow cytometry testing of human U-87 MG cells with Hemoglobin subunit gamma-2 antibody; Blue=isotype control, Green= Hemoglobin subunit gamma-2 antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Hemoglobin subunit gamma-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Gamma chains make up the fetal hemoglobin F, in combination with alpha chains. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 101-133 from the human protein was used as the immunogen for the Hemoglobin subunit gamma-2 antibody.
Limitation:
This Hemoglobin subunit gamma-2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P69892