COL5A1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54339
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54339-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54339-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the COL5A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human uterus tissue with COL5A1 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human heart tissue with COL5A1 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
3 / 5
Western blot testing of human U251 cell lysate with COL5A1 antibody. Predicted molecular weight ~184 kDa but can be observed at up to ~250 kDa.
4 / 5
Western blot testing of mouse bladder lysate with COL5A1 antibody. Predicted molecular weight ~184 kDa but can be observed at up to ~250 kDa.
5 / 5
Western blot testing of human HeLa cell lysate with COL5A1 antibody. Predicted molecular weight ~184 kDa but can be observed at up to ~250 kDa.
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IHC testing of FFPE human uterus tissue with COL5A1 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human heart tissue with COL5A1 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Western blot testing of human U251 cell lysate with COL5A1 antibody. Predicted molecular weight ~184 kDa but can be observed at up to ~250 kDa.
Western blot testing of mouse bladder lysate with COL5A1 antibody. Predicted molecular weight ~184 kDa but can be observed at up to ~250 kDa.
Western blot testing of human HeLa cell lysate with COL5A1 antibody. Predicted molecular weight ~184 kDa but can be observed at up to ~250 kDa.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the COL5A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
COL5A1 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. COL5A1 product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 54-82 from the human protein was used as the immunogen for the COL5A1 antibody.
Limitation:
This COL5A1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P20908