LXRB Antibody / NR1H2

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NSJ Bioreagents
Product Code: NSJ-RQ6202
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6202-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the LXR beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human MCF7 cells with LXR beta antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human U-2 OS cells with LXR beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LXR beta antibody.
3 / 3
Western blot testing of 1) human PC-3, 2) human HL60, 3) mouse liver and 4) mouse RAW264.7 lysate with LXR beta antibody. Predicted molecular weight ~51 kDa.
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Immunofluorescent staining of FFPE human MCF7 cells with LXR beta antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-2 OS cells with LXR beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LXR beta antibody.
Western blot testing of 1) human PC-3, 2) human HL60, 3) mouse liver and 4) mouse RAW264.7 lysate with LXR beta antibody. Predicted molecular weight ~51 kDa.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the LXR beta antibody should be determined by the researcher.
Description:
Liver X receptor beta (LXR-b) is a member of the nuclear receptor family of transcription factors. LXR-b is encoded by the NR1H2 gene (nuclear receptor subfamily 1, group H, member 2). The liver X receptors, LXRA and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors and regulate expression of target genes containing LXR response elements.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids K31-A342) was used as the immunogen for the LXR beta antibody.
Limitation:
This LXR beta antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P55055