Cyclophilin E Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6110
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6110-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Cyclophilin E antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A431 cells with Cyclophilin E antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Immunofluorescent staining of FFPE human PC-3 cells with Cyclophilin E antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 4
Western blot testing of human 1) HEK293, 2) HeLa, 3) K562, 4) PC-3, 5) Caco-2 and 6) mouse HEPA1-6 lysate with Cyclophilin E antibody. Predicted molecular weight ~33 kDa.
4 / 4
Flow cytometry testing of human ThP-1 cells with Cyclophilin E antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cyclophilin E antibody.
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Immunofluorescent staining of FFPE human A431 cells with Cyclophilin E antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human PC-3 cells with Cyclophilin E antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HEK293, 2) HeLa, 3) K562, 4) PC-3, 5) Caco-2 and 6) mouse HEPA1-6 lysate with Cyclophilin E antibody. Predicted molecular weight ~33 kDa.
Flow cytometry testing of human ThP-1 cells with Cyclophilin E antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cyclophilin E antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Cyclophilin E antibody should be determined by the researcher.
Description:
Peptidylprolyl isomerase E (cyclophilin E), also known as PPIE, is an enzyme which in humans is encoded by the PPIE gene on chromosome 1. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids M1-V301) was used as the immunogen for the Cyclophilin E antibody.
Limitation:
This Cyclophilin E antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9UNP9