Glutaredoxin 2 Antibody / GLRX2

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NSJ Bioreagents
Product Code: NSJ-RQ6094
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6094-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the GLRX2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 10
IHC staining of FFPE human testis cancer with GLRX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 10
IHC staining of FFPE human bladder cancer with GLRX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 10
IHC staining of FFPE human renal carcinoma with GLRX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 10
IHC staining of FFPE human ovarian cancer with GLRX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE human gastric cancer with GLRX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE mouse brain with GLRX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE mouse brain with GLRX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 10
Immunofluorescent staining of FFPE human A431 cells with GLRX2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
9 / 10
Western blot testing of human 1) K562, 2) U-87 MG, 3) PC-3, 4) HepG2 and 5) mouse thymus lysate with GLRX2 antibody. Predicted molecular weight ~18 kDa.
10 / 10
Flow cytometry testing of human Caco-2 cells with GLRX2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GLRX2 antibody.
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IHC staining of FFPE human testis cancer with GLRX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder cancer with GLRX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal carcinoma with GLRX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer with GLRX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric cancer with GLRX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with GLRX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with GLRX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A431 cells with GLRX2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) K562, 2) U-87 MG, 3) PC-3, 4) HepG2 and 5) mouse thymus lysate with GLRX2 antibody. Predicted molecular weight ~18 kDa.
Flow cytometry testing of human Caco-2 cells with GLRX2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GLRX2 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the GLRX2 antibody should be determined by the researcher.
Description:
GLRX2 (Glutaredoxin-2) also known as Glutaredoxin-2, mitochondrial or GRX2, is a protein that in humans is encoded by the GLRX2 gene. Glutaredoxins(e.g., GLRX) are a family of glutathione-dependent hydrogen donors that participate in a variety of cellular redox reactions. By sequence analysis, Lundberg et al.(2001) and Gladyshev et al.(2001) identified the GLRX2 gene within a clone mapping to chromosome 1q31.2-q31.3. Lundberg et al.(2001) determined that the GLRX2 gene contains 5 exons spanning about 9.6 kb. The GLRX2B transcript uses a first exon(exon 1B) located upstream from the first exon used by the GLRX2A transcript(exon 1A), suggesting that alternative splicing generates the isoforms. Lundberg et al.(2001)assayed reductase activity in recombinant proteins of both GLRX2 isoforms. They found that both have GSH-dependent dehydroascorbate reductase activity and 2-hydroxyethyl disulfide reductase activity.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids M1-Q164) was used as the immunogen for the GLRX2 antibody.
Limitation:
This GLRX2 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9NS18