Lanosterol 14-alpha demethylase Antibody / CYP51A1

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NSJ Bioreagents
Product Code: NSJ-RQ6417
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6417-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Lanosterol 14-alpha demethylase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human Caco-2 cells with Lanosterol 14-alpha demethylase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) monkey COS-7, 2) human A431, 3) human K562, 4) mouse liver and 5) mouse kidney tissue lysate with Lanosterol 14-alpha demethylase antibody. Predicted molecular weight ~57 kDa.
3 / 3
Flow cytometry testing of human HepG2 cells with Lanosterol 14-alpha demethylase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Lanosterol 14-alpha demethylase antibody.
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Immunofluorescent staining of FFPE human Caco-2 cells with Lanosterol 14-alpha demethylase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) monkey COS-7, 2) human A431, 3) human K562, 4) mouse liver and 5) mouse kidney tissue lysate with Lanosterol 14-alpha demethylase antibody. Predicted molecular weight ~57 kDa.
Flow cytometry testing of human HepG2 cells with Lanosterol 14-alpha demethylase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Lanosterol 14-alpha demethylase antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Lanosterol 14-alpha demethylase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. Several P450 enzymes have been classified by sequence similarities as members of the CYP1A and CYP2A subfamilies. CYP51A1 (cytochrome P450, family 51, subfamily A, polypeptide 1), also known as LDM (lanosterol 14-alpha demethylase) or cytochrome P450-14DM, is a 503 amino acid protein localized to endoplasmic reticulum membrane. CYP51A1 is an important enzyme for zymosterol and steroid biosynthesis. CYP51A1 catalyzes C14-demethylation of lanosterol, transforming lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. CYP51A1 is ubiquitously expressed, with highest levels found in liver, ovary, testis, lung, kidney and prostate.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K141-K503) was used at the immunogen for the Lanosterol 14-alpha demethylase antibody.
Limitation:
This Lanosterol 14-alpha demethylase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Monkey
Uniprot #:
Q16850