ATPase subunit F6 Antibody / ATP5PF

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NSJ Bioreagents
Product Code: NSJ-F54650
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54650-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54650-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ATPase subunit F6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of mouse stomach tissue lysate with ATPase subunit F6 antibody. Predicted molecular weight ~13 kDa.
2 / 4
Western blot testing of rat heart tissue lysate with ATPase subunit F6 antibody. Predicted molecular weight ~13 kDa.
3 / 4
Immunofluorescent staining of human U-251 MG cells with ATPase subunit F6 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
IHC testing of FFPE human liver tissue with ATPase subunit F6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of mouse stomach tissue lysate with ATPase subunit F6 antibody. Predicted molecular weight ~13 kDa.
Western blot testing of rat heart tissue lysate with ATPase subunit F6 antibody. Predicted molecular weight ~13 kDa.
Immunofluorescent staining of human U-251 MG cells with ATPase subunit F6 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human liver tissue with ATPase subunit F6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the ATPase subunit F6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex, required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. A pseudogene exists on chromosome Yp11.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 28-56 from the human protein was used as the immunogen for the ATPase subunit F6 antibody.
Limitation:
This ATPase subunit F6 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P18859