CA13 Antibody / Carbonic Anhydrase XIII

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NSJ Bioreagents
Product Code: NSJ-RQ6086
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6086-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CA13 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 7
Immunofluorescent staining of FFPE human HepG2 cells with CA13 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 7
IHC staining of FFPE human placenta with CA13 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE human ovarian cancer with CA13 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human liver cancer with CA13 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE human melanoma with CA13 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Western blot testing of human 1) HepG2, 2) Caco-2, 3) U-87 MG, 4) K562, 5) HeLa, 6) rat intestine and 7) mouse NIH 3T3 lysate with CA13 antibody. Predicted molecular weight ~29 kDa.
7 / 7
Flow cytometry testing of human Caco-2 cells with CA13 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CA13 antibody.
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Immunofluorescent staining of FFPE human HepG2 cells with CA13 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human placenta with CA13 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer with CA13 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with CA13 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human melanoma with CA13 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HepG2, 2) Caco-2, 3) U-87 MG, 4) K562, 5) HeLa, 6) rat intestine and 7) mouse NIH 3T3 lysate with CA13 antibody. Predicted molecular weight ~29 kDa.
Flow cytometry testing of human Caco-2 cells with CA13 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CA13 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the CA13 antibody should be determined by the researcher.
Description:
Carbonic anhydrase 13 is a protein that in humans is encoded by the CA13 gene. Carbonic anhydrases (CAs) are a family of zinc metalloenzymes that catalyze the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids RLSWGYREHNGPIHWKEFFP from the human protein were used as the immunogen for the CA13 antibody.
Limitation:
This CA13 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q8N1Q1