GABARAP Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6177
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6177-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the GABARAP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human ovarian cancer with GABARAP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human renal cancer with GABARAP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human esophageal squamous cancer with GABARAP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse brain with GABARAP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat brain with GABARAP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Western blot testing of human 1) HEK293, 2) Jurkat, 3) A549, 4) HeLa, 5) U-87 MG, 6) HepG2, 7) U937, 8) rat liver, 9) rat pancreas, 10) mouse liver and 11) mouse pancreas lysate with GABARAP antibody. Predicted molecular weight: 14-16 kDa.
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Flow cytometry testing of human U-251 cells with GABARAP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GABARAP antibody.
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Flow cytometry testing of mouse HEPA1-6 cells with GABARAP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GABARAP antibody.
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IHC staining of FFPE human ovarian cancer with GABARAP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cancer with GABARAP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous cancer with GABARAP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with GABARAP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with GABARAP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HEK293, 2) Jurkat, 3) A549, 4) HeLa, 5) U-87 MG, 6) HepG2, 7) U937, 8) rat liver, 9) rat pancreas, 10) mouse liver and 11) mouse pancreas lysate with GABARAP antibody. Predicted molecular weight: 14-16 kDa.
Flow cytometry testing of human U-251 cells with GABARAP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GABARAP antibody.
Flow cytometry testing of mouse HEPA1-6 cells with GABARAP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GABARAP antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the GABARAP antibody should be determined by the researcher.
Description:
Gamma-aminobutyric acid receptor-associated protein is a protein that in humans is encoded by the GABARAP gene. Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids MKFVYKEEHPFEKRRSE from the human protein were used as the immunogen for the GABARAP antibody.
Limitation:
This GABARAP antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O95166