Synaptic vesicle glycoprotein 2A Antibody / SV2A

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NSJ Bioreagents
Product Code: NSJ-RQ6342
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6342-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Synaptic vesicle glycoprotein 2A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human rectal cancer with Synaptic vesicle glycoprotein 2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human ovarian serous adenocarcinoma with Synaptic vesicle glycoprotein 2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human liver cancer tissue with Synaptic vesicle glycoprotein 2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung cancer tissue with Synaptic vesicle glycoprotein 2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse brain tissue with Synaptic vesicle glycoprotein 2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat brain tissue with Synaptic vesicle glycoprotein 2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Western blot testing of 1) rat brain and 2) mouse brain lysate with Synaptic vesicle glycoprotein 2A antibody. Predicted molecular weight ~83 kDa but may be observed at larger sizes due to glycosylation.
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Flow cytometry testing of human U-2 OS cells with Synaptic vesicle glycoprotein 2A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Synaptic vesicle glycoprotein 2A antibody.
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IHC staining of FFPE human rectal cancer with Synaptic vesicle glycoprotein 2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous adenocarcinoma with Synaptic vesicle glycoprotein 2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Synaptic vesicle glycoprotein 2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Synaptic vesicle glycoprotein 2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with Synaptic vesicle glycoprotein 2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Synaptic vesicle glycoprotein 2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat brain and 2) mouse brain lysate with Synaptic vesicle glycoprotein 2A antibody. Predicted molecular weight ~83 kDa but may be observed at larger sizes due to glycosylation.
Flow cytometry testing of human U-2 OS cells with Synaptic vesicle glycoprotein 2A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Synaptic vesicle glycoprotein 2A antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Synaptic vesicle glycoprotein 2A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Synaptic vesicle glycoprotein 2A is a ubiquitous synaptic vesicle protein that in humans is encoded by the SV2A gene. The protein encoded by this gene is one of three related synaptic vesicle proteins. The encoded protein may interact with synaptotagmin to enhance low frequency neurotransmission in quiescent neurons.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids MEEGFRDRAAFIRGAKDIAKEVKKHAAKKVVK were used as the immunogen for the Synaptic vesicle glycoprotein 2A antibody.
Limitation:
This Synaptic vesicle glycoprotein 2A antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q7L0J3