IRAK1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5587
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5587-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the IRAK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human ovarian cancer with IRAK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human ovarian cancer with IRAK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 6
Western blot testing of human 1) PC-3, 2) U-2 OS, 3) HEK293 and 4) K562 cell lysate with IRAK1 antibody. Predicted molecular weight 68-76 kDa.
4 / 6
Western blot testing of 1) rat brain, 2) rat liver, 3) mouse brain and 4) mouse Neuro-2a lysate with IRAK1 antibody. Predicted molecular weight 68-76 kDa.
5 / 6
Flow cytometry testing of human A549 cells with IRAK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IRAK1 antibody.
6 / 6
Immunofluorescent staining of FFPE human K562 cells with IRAK1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC staining of FFPE human ovarian cancer with IRAK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer with IRAK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of human 1) PC-3, 2) U-2 OS, 3) HEK293 and 4) K562 cell lysate with IRAK1 antibody. Predicted molecular weight 68-76 kDa.
Western blot testing of 1) rat brain, 2) rat liver, 3) mouse brain and 4) mouse Neuro-2a lysate with IRAK1 antibody. Predicted molecular weight 68-76 kDa.
Flow cytometry testing of human A549 cells with IRAK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IRAK1 antibody.
Immunofluorescent staining of FFPE human K562 cells with IRAK1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the IRAK1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Interleukin-1 receptor-associated kinase 1, also called IRAK1, is an enzyme that in humans is encoded by the IRAK1 gene. By radiation hybrid analysis, this gene is mapped to chromosome Xq28. Serine/threonine-protein kinase plays a critical role in initiating innate immune response against foreign pathogens. This gene involved in Toll-like receptor (TLR) and IL-1R signaling pathways. This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids NRNARVADLVHILTHLQLLRARD were used as the immunogen for the IRAK1 antibody.
Limitation:
This IRAK1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P51617