LPP Antibody / Lipoma preferred partner

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NSJ Bioreagents
Product Code: NSJ-RQ5738
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5738-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the LPP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE human intestinal cancer with LPP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
2 / 5
IHC staining of FFPE human rectal cancer with LPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human breast cancer with LPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
IHC staining of FFPE rat intestine with LPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 5
Western blot testing of human 1) A549, 2) HepG2, 3) HeLa, 4) MDA-MB-453, 5) monkey COS-7, 6) rat ovary, 7) rat PC-12 and 8) mouse ovary lysate with LPP antibody. Predicted molecular weight: ~66 kDa but is routinely observed at 80~85 kDa.
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Immunofluorescent staining of FFPE human intestinal cancer with LPP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
IHC staining of FFPE human rectal cancer with LPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with LPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with LPP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) A549, 2) HepG2, 3) HeLa, 4) MDA-MB-453, 5) monkey COS-7, 6) rat ovary, 7) rat PC-12 and 8) mouse ovary lysate with LPP antibody. Predicted molecular weight: ~66 kDa but is routinely observed at 80~85 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the LPP antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
LPP (Lim Domain-Containing Preferred Translocation Partner in Lipoma), is a protein that in humans is encoded by the LPP gene. Petit et al. (1996) commented that the LPP-encoded protein should be classified as a novel member of the group 3 proteins of the LIM protein gene family. By partial cDNA cloning, Petit et al. (1996) established features of the genetic organization of LPP. The gene was found to span a genomic region of over 400 kb. By FISH and Southern blot analyses, Daheron et al. (2001) identified a rearrangement in the mixed lineage leukemia gene due to a novel t(3;11)(q28;q23) translocation in a patient who developed acute myeloid leukemia of the M5 type 3 years after treatment for a follicular lymphoma.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KTCNSARIRVLTAKASTDL from the human protein were used as the immunogen for the LPP antibody.
Limitation:
This LPP antibody is available for research use only.
Localization:
Nuclear, cytoplasmic, plasma membrane
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q93052