LSM2 Antibody

Product Code: NSJ-RQ5825

Code	Size	Price

NSJ-RQ5825-100ug

100 ug

£535.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species:

Human
Mouse
Rat

Applications:

Fluorescence-activated cell sorting (FACS)
Immunofluorescence (IF)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

After reconstitution, the LSM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 13

Immunofluorescent staining of FFPE human MCF7 cells with LSM2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

2 / 13

IHC staining of FFPE human appendicitis tissue with LSM2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

3 / 13

IHC staining of FFPE human adrenal carcinoma with LSM2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

4 / 13

IHC staining of FFPE human lung cancer with LSM2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

5 / 13

IHC staining of FFPE human rectal cancer with LSM2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

6 / 13

IHC staining of FFPE human thyroid cancer with LSM2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

7 / 13

IHC staining of FFPE human liver cancer with LSM2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

8 / 13

IHC staining of FFPE human breast cancer with LSM2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

9 / 13

IHC staining of FFPE mouse lung with LSM2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

10 / 13

IHC staining of FFPE rat lung with LSM2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

11 / 13

Western blot testing of human 1) SW620, 2) HEK293, 3) K562, 4) Caco-2, 5) monkey COS-7, 6) Raji, 7) A431 and 8) U-2 OS lysate with LSM2 antibody. Predicted molecular weight: ~10 kDa.

12 / 13

Western blot testing of rat 1) brain, 2) testis, 3) small intestine, 4) C6 and mouse 5) testis, 6) Neuro-2a and 7) HEPA1-6 lysate with LSM2 antibody. Predicted molecular weight: ~10 kDa.

13 / 13

Flow cytometry testing of human K562 cells with LSM2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LSM2 antibody.

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Documents

Further Information

Application Details :

Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells

Application Note:

Optimal dilution of the LSM2 antibody should be determined by the researcher.

Buffer:

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Description:

U6 snRNA-associated Sm-like protein LSm2 is a protein that in humans is encoded by the LSM2 gene. This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 19.

Format :

Antigen affinity purified

Formulation :

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Immunogen:

Amino acids DTQLLQDAARKEALQQKQ from the human protein were used as the immunogen for the LSM2 antibody.

Limitation:

This LSM2 antibody is available for research use only.

Purity:

Affinity purified

Species Reactivity :

Human, Mouse, Rat

Uniprot #:

Q9Y333