MC4R Antibody / MC4-R / Melanocortin Receptor 4

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NSJ Bioreagents
Product Code: NSJ-RQ6059
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6059-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MC4-R antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human A431 cells with MC4-R antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC staining of FFPE human rectal cancer with MC4-R antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) human U-87 MG, 2) human HEK293, 3) rat PC-12 and 4) mouse Neuro-2a cell lysate with MC4-R antibody. Predicted molecular weight ~37 kDa.
4 / 4
Flow cytometry testing of human U937 cells with MC4-R antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MC4-R antibody.
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Immunofluorescent staining of FFPE human A431 cells with MC4-R antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human rectal cancer with MC4-R antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human U-87 MG, 2) human HEK293, 3) rat PC-12 and 4) mouse Neuro-2a cell lysate with MC4-R antibody. Predicted molecular weight ~37 kDa.
Flow cytometry testing of human U937 cells with MC4-R antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MC4-R antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the MC4-R antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Melanocortin receptor 4 is a protein that in humans is encoded by the MC4R gene. This gene is mapped to 18q21.32. The protein encoded by this gene is a membrane-bound receptor and member of the melanocortin receptor family. The encoded protein interacts with adrenocorticotropic and MSH hormones and is mediated by G proteins. This is an intronless gene. Defects in this gene are a cause of autosomal dominant obesity.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids QELRKTFKEIICCYPLGGLCDLSSRY from the human protein were used as the immunogen for the MC4-R antibody.
Limitation:
This MC4-R antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P32245