Sclerostin Antibody / SOST

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NSJ Bioreagents
Product Code: NSJ-F54329
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54329-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54329-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Sclerostin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) kidney and 2) liver lysate with Sclerostin antibody. Predicted molecular weight ~24 kDa.
2 / 4
Western blot testing of 1) mouse lung, 2) human liver and 3) human HeLa lysate with Sclerostin antibody. Predicted molecular weight ~24 kDa.
3 / 4
Western blot testing of 1) mouse liver, 2) mouse lung and 3) rat kidney lysate with Sclerostin antibody. Predicted molecular weight ~24 kDa.
4 / 4
IHC testing of FFPE human kidney tissue with Sclerostin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) kidney and 2) liver lysate with Sclerostin antibody. Predicted molecular weight ~24 kDa.
Western blot testing of 1) mouse lung, 2) human liver and 3) human HeLa lysate with Sclerostin antibody. Predicted molecular weight ~24 kDa.
Western blot testing of 1) mouse liver, 2) mouse lung and 3) rat kidney lysate with Sclerostin antibody. Predicted molecular weight ~24 kDa.
IHC testing of FFPE human kidney tissue with Sclerostin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Sclerostin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 12-42 from the human protein was used as the immunogen for the Sclerostin antibody.
Limitation:
This Sclerostin antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9BQB4