ADP-ribosylation factor 6 Antibody / ARF6

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ6368
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6368-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the ADP-ribosylation factor 6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human A431 cells with ADP-ribosylation factor 6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of 1) human HeLa, 2) human PC-3, 3) human U-2 OS, 4) rat liver, 5) mouse lung and 6) mouse liver lysate with ADP-ribosylation factor 6 antibody. Predicted molecular weight ~20 kDa.
3 / 4
Flow cytometry testing of human PC-3 cells with ADP-ribosylation factor 6 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADP-ribosylation factor 6 antibody.
4 / 4
Flow cytometry testing of rat RH35 cells with ADP-ribosylation factor 6 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADP-ribosylation factor 6 antibody.
❮❯

Immunofluorescent staining of FFPE human A431 cells with ADP-ribosylation factor 6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human PC-3, 3) human U-2 OS, 4) rat liver, 5) mouse lung and 6) mouse liver lysate with ADP-ribosylation factor 6 antibody. Predicted molecular weight ~20 kDa.
Flow cytometry testing of human PC-3 cells with ADP-ribosylation factor 6 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADP-ribosylation factor 6 antibody.
Flow cytometry testing of rat RH35 cells with ADP-ribosylation factor 6 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADP-ribosylation factor 6 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence (FFPE): 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ADP-ribosylation factor 6 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
ADP-ribosylation factor 6 (ARF6) is a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R75-S175) was used as the immunogen for the ADP-ribosylation factor 6 antibody.
Limitation:
This ADP-ribosylation factor 6 antibody is available for research use only.
Localization:
Cytoplasmic, cell membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P62330