STK39 Antibody / SPAK

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NSJ Bioreagents
Product Code: NSJ-RQ6211
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6211-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the STK39 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human Daudi, 2) human HL60, 3) rat brain, 4) rat testis, 5) mouse brain and 6) mouse testis lysate with STK39 antibody. Predicted molecular weight ~60 kDa.
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Immunofluorescent staining of FFPE human A431 cells with STK39 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human HL60 cells with STK39 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= STK39 antibody.
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Western blot testing of 1) human Daudi, 2) human HL60, 3) rat brain, 4) rat testis, 5) mouse brain and 6) mouse testis lysate with STK39 antibody. Predicted molecular weight ~60 kDa.
Immunofluorescent staining of FFPE human A431 cells with STK39 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human HL60 cells with STK39 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= STK39 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the STK39 antibody should be determined by the researcher.
Description:
STE20/SPS1-related proline-alanine-rich protein kinase is an enzyme that in humans is encoded by the STK39 gene. This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids Q338-S545) was used as the immunogen for the STK39 antibody.
Limitation:
This STK39 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UEW8