BRD4 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6454
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6454-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the BRD4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human 293T cells with BRD4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BRD4 antibody.
2 / 2
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with BRD4 antibody. Predicted molecular weight ~156 kDa (long form), but has been observed at 200+ kDa.
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Flow cytometry testing of human 293T cells with BRD4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BRD4 antibody.
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with BRD4 antibody. Predicted molecular weight ~156 kDa (long form), but has been observed at 200+ kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the BRD4 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Bromodomain-containing protein 4 is a protein that in humans is encoded by the BRD4 gene. The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A802-R1163) was used at the immunogen for the BRD4 antibody. The antibody is not suitable for the detection of the short form of the protein.
Limitation:
This BRD4 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O60885