Breakpoint cluster region protein Antibody / BCR

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ6452
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6452-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Breakpoint cluster region protein antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Flow cytometry testing of human K562 cells with Breakpoint cluster region protein antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Breakpoint cluster region protein antibody.
2 / 3
Flow cytometry testing of human HeLa cells with Breakpoint cluster region protein antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Breakpoint cluster region protein antibody.
3 / 3
Western blot testing of 1) human Jurkat, 2) human K562, 3) human HEK293, 4) rat brain and 5) mouse brain tissue lysate with Breakpoint cluster region protein antibody. Expected molecular weight: 130-190 kDa (multiple isoforms).
❮❯

Flow cytometry testing of human K562 cells with Breakpoint cluster region protein antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Breakpoint cluster region protein antibody.
Flow cytometry testing of human HeLa cells with Breakpoint cluster region protein antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Breakpoint cluster region protein antibody.
Western blot testing of 1) human Jurkat, 2) human K562, 3) human HEK293, 4) rat brain and 5) mouse brain tissue lysate with Breakpoint cluster region protein antibody. Expected molecular weight: 130-190 kDa (multiple isoforms).

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Breakpoint cluster region protein antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The breakpoint cluster region protein (BCR) is a protein that in humans is encoded by the BCR gene. A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids D3-N264) was used as the immunogen for the Breakpoint cluster region protein antibody.
Limitation:
This Breakpoint cluster region protein antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P11274