CD154 Antibody / CD40L / TRAP

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NSJ Bioreagents
Product Code: NSJ-RQ6411
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6411-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the CD154 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Flow cytometry testing of human PBM cells with CD154 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD154 antibody.
2 / 3
Flow cytometry testing of rat C6 cells with CD154 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD154 antibody.
3 / 3
Western blot testing of human 1) HL60, 2) K562, 3) Raji, 4) Jurkat, 5) rat RH35, 6) mouse spleen and 7) mouse ANA-1 cell lysate with CD154 antibody. Expected molecular weight: 29-39 kDa (depending on glycosylation level) or ~18 kDa (soluble form).
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Flow cytometry testing of human PBM cells with CD154 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD154 antibody.
Flow cytometry testing of rat C6 cells with CD154 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD154 antibody.
Western blot testing of human 1) HL60, 2) K562, 3) Raji, 4) Jurkat, 5) rat RH35, 6) mouse spleen and 7) mouse ANA-1 cell lysate with CD154 antibody. Expected molecular weight: 29-39 kDa (depending on glycosylation level) or ~18 kDa (soluble form).

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CD154 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
CD154 (CD40 ligand) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD154 with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since it is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb /IIIa receptor in patients with acute coronary syndromes was investigated. The soluble for of the protein may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells. CD154 plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E76-H249) was used at the immunogen for the CD154 antibody.
Limitation:
This CD154 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P29965