cPLA2-beta Antibody / PLA2G4B

NSJ Bioreagents
Product Code: NSJ-RQ6334
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6334-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the cPLA2-beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 2
Flow cytometry testing of human HeLa cells with cPLA2-beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= cPLA2-beta antibody.
2 / 2
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with cPLA2-beta antibody. Predicted molecular weight ~88 kDa.

Flow cytometry testing of human HeLa cells with cPLA2-beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= cPLA2-beta antibody.
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with cPLA2-beta antibody. Predicted molecular weight ~88 kDa.

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the cPLA2-beta antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Phospholipase A2, group IVB (cytosolic), also known as PLA2G4B, is a human gene. This gene encodes a member of the cytosolic phospholipase A2 protein family. Phospholipase A2 enzymes hydrolyze the sn-2 bond of phospholipids, releasing lysophospholipids and fatty acids. This enzyme may be associated with mitochondria and early endosomes. Most tissues also express read-through transcripts from the upstream gene into this gene, some of which may encode fusion proteins combining the N-terminus of the upstream gene including its JmjC domain with the almost complete coding region of this gene, including the C2 and cytoplasmic phospholipase A2 domains.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids N61-H781) was used as the immunogen for the cPLA2-beta antibody.
Limitation:
This cPLA2-beta antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P0C869