EPS15 Antibody / Epidermal growth factor receptor substrate 15

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NSJ Bioreagents
Product Code: NSJ-RQ5974
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5974-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the EPS15 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human A431 cells with EPS15 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EPS15 antibody.
2 / 2
Western blot testing of 1) rat brain, 2) mouse brain, 3) mouse NIH 3T3 and human 4) HeLa, 5) Jurkat, 6) HEK293, 7) PC-3 and 8) ThP1 lysate with EPS15 antibody. Predicted molecular weight ~99 kDa, routinely observed at 120-150 kDa.
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Flow cytometry testing of human A431 cells with EPS15 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EPS15 antibody.
Western blot testing of 1) rat brain, 2) mouse brain, 3) mouse NIH 3T3 and human 4) HeLa, 5) Jurkat, 6) HEK293, 7) PC-3 and 8) ThP1 lysate with EPS15 antibody. Predicted molecular weight ~99 kDa, routinely observed at 120-150 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the EPS15 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Epidermal growth factor receptor substrate 15 is a protein that in humans is encoded by the EPS15 gene. It is mapped to 1p32.3. This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-K295) was used as the immunogen for the EPS15 antibody.
Limitation:
This EPS15 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P42566