Helios Antibody / ZNFN1A2 / IKZF2

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NSJ Bioreagents
Product Code: NSJ-RQ6055
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6055-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Helios antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human HL60 cells with Helios antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Helios antibody.
2 / 2
Western blot testing of human 1) HeLa, 2) K562, 3) Jurkat, 4) SW579, 5) A549, 6) rat spleen and 7) mouse ANA-1 cell lysate with Helios antibody. Expected molecular weight: 60-70 kDa.
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Flow cytometry testing of human HL60 cells with Helios antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Helios antibody.
Western blot testing of human 1) HeLa, 2) K562, 3) Jurkat, 4) SW579, 5) A549, 6) rat spleen and 7) mouse ANA-1 cell lysate with Helios antibody. Expected molecular weight: 60-70 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Helios antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Zinc finger protein Helios is a protein that in humans is encoded by the IKZF2 gene. This gene is mapped to 2q34.This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-R477) was used as the immunogen for the Helios antibody.
Limitation:
This Helios antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UKS7