Myb-related protein A Antibody / A-Myb / MYBL1

NSJ Bioreagents
Product Code: NSJ-RQ6429
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6429-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Myb-related protein A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 2
Flow cytometry testing of human U-87 MG cells with Myb-related protein A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Myb-related protein A antibody.
2 / 2
Western blot testing of human 1) T-47D, 2) A431, 3) PC-3, 4) U-87 MG, 5) A549, 6) rat stomach and 7) mouse stomach tissue lysate with Myb-related protein A antibody. Predicted molecular weight ~86 kDa.

Flow cytometry testing of human U-87 MG cells with Myb-related protein A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Myb-related protein A antibody.
Western blot testing of human 1) T-47D, 2) A431, 3) PC-3, 4) U-87 MG, 5) A549, 6) rat stomach and 7) mouse stomach tissue lysate with Myb-related protein A antibody. Predicted molecular weight ~86 kDa.

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Myb-related protein A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Myb-related protein A is a protein that in humans is encoded by the MYBL1 gene. A-Myb is expressed at specific times in reproductive tissues, some neural cells, and a subset of normal and neoplastic B lymphocytes. Both A-Myb and B-Myb are expressed in t(14:18) lymphoma cells where they then inhibit cell arrest and apoptotic signaling. Expression of B-Myb rescues cells from p53-induced G1 phase arrest that is mediated by p21, while A-Myb functions as an anti-apoptotic factor by effectively activating the bcl-2 promoter and thereby up-regulating Bcl-2 expression.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K199-L752) was used as the immunogen for the Myb-related protein A antibody.
Limitation:
This Myb-related protein A antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P10243