PAX1 Antibody / Paired box 1

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NSJ Bioreagents
Product Code: NSJ-RQ5906
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5906-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the PAX1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human 293T cells with PAX1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PAX1 antibody.
2 / 2
Western blot testing of human 1) placenta, 2) SW579, 3) A431, 4) U-2 OS, 5) rat thymus, 6) rat ovary, 7) rat lung, 8) mouse thymus, 9) mouse ovary, 10) mouse lung and 11) mouse SP2/0 lysate with PAX1 antibody. Expected molecular weight: 45-55 kDa.
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Flow cytometry testing of human 293T cells with PAX1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PAX1 antibody.
Western blot testing of human 1) placenta, 2) SW579, 3) A431, 4) U-2 OS, 5) rat thymus, 6) rat ovary, 7) rat lung, 8) mouse thymus, 9) mouse ovary, 10) mouse lung and 11) mouse SP2/0 lysate with PAX1 antibody. Expected molecular weight: 45-55 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PAX1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. This gene plays a role in pattern formation during embryogenesis and may be essential for development of the vertebral column. This gene is silenced by methylation in ovarian and cervical cancers and may be a tumor suppressor gene. Mutations in this gene are also associated with vertebral malformations.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids S218-P382) was used as the immunogen for the PAX1 antibody.
Limitation:
This PAX1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P15863