PIAS2 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6001
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6001-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the PIAS2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human U937 cells with PIAS2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PIAS2 antibody.
2 / 2
Western blot testing of human 1) K562, 2) HeLa, 3) HepG2, 4) HEK293, 5) HL-60, 6) Raji, 7) A431, 8) rat brain, 9) rat C6, 10) mouse brain, 11) mouse testis, 12) mouse Neuro-2a and 13) mouse RAW264.7 lysate with PIAS2 antibody. Predicted molecular weight: ~63 kDa.
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Flow cytometry testing of human U937 cells with PIAS2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PIAS2 antibody.
Western blot testing of human 1) K562, 2) HeLa, 3) HepG2, 4) HEK293, 5) HL-60, 6) Raji, 7) A431, 8) rat brain, 9) rat C6, 10) mouse brain, 11) mouse testis, 12) mouse Neuro-2a and 13) mouse RAW264.7 lysate with PIAS2 antibody. Predicted molecular weight: ~63 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PIAS2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
E3 SUMO-protein ligase PIAS2 is an enzyme that in humans is encoded by the PIAS2 gene. It is mapped to 18q21.1. This gene encodes a member of the protein inhibitor of activated STAT family, which function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E93-K311) was used as the immunogen for the PIAS2 antibody.
Limitation:
This PIAS2 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O75928