Pinin Antibody / PNN / DRSP

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NSJ Bioreagents
Product Code: NSJ-RQ5834
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5834-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Pinin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human HepG2 cells with Pinin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Pinin antibody.
2 / 3
Flow cytometry testing of human HL-60 cells with Pinin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Pinin antibody.
3 / 3
Western blot testing of human 1) HeLa, 2) HepG2, 3) HL-60, 4) K562, 5) rat liver, 6) rat kidney, 7) mouse Neuro-2a lysate with Pinin antibody. Predicted molecular weight ~82 kDa.
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Flow cytometry testing of human HepG2 cells with Pinin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Pinin antibody.
Flow cytometry testing of human HL-60 cells with Pinin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Pinin antibody.
Western blot testing of human 1) HeLa, 2) HepG2, 3) HL-60, 4) K562, 5) rat liver, 6) rat kidney, 7) mouse Neuro-2a lysate with Pinin antibody. Predicted molecular weight ~82 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Pinin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Pinin is a protein that in humans is encoded by the PNN gene. By yeast 2-hybrid analysis of HeLa cells, followed by sequence analysis, it was found that epitope-tagged human PNN interacted with the serine/arginine (SR)-rich proteins SRP75/SRSF4, SRM300/SRRM2, and SRRP130/PNISR. Truncation analysis revealed that the polyserine/RS domain of PNN and flanking sequences participated in binding to these SR proteins. The 4 proteins colocalized in the nucleus of HCE-T cells, and overexpression of any of these proteins affected the distribution of the others between nuclear speckles and nucleoplasm.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A2-L241) was used as the immunogen for the Pinin antibody.
Limitation:
This Pinin antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9H307