AMPK alpha 1 Antibody / PRKAA1

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NSJ Bioreagents
Product Code: NSJ-RQ6111
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6111-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the PRKAA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human Jurkat, 2) human HeLa, 3) human MCF7, 4) monkey COS-7, 5) human K562, 6) HEK293, 7) rat brain, 8) rat heart, 9) mouse brain, 10) mouse heart and 11) mouse intestine lysate with PRKAA1 antibody. Predicted molecular weight: 64-66 kDa (two isoforms).
2 / 2
Flow cytometry testing of human SiHa cells with PRKAA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRKAA1 antibody.
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Western blot testing of 1) human Jurkat, 2) human HeLa, 3) human MCF7, 4) monkey COS-7, 5) human K562, 6) HEK293, 7) rat brain, 8) rat heart, 9) mouse brain, 10) mouse heart and 11) mouse intestine lysate with PRKAA1 antibody. Predicted molecular weight: 64-66 kDa (two isoforms).
Flow cytometry testing of human SiHa cells with PRKAA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRKAA1 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PRKAA1 antibody should be determined by the researcher.
Description:
5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids D359-P539) was used as the immunogen for the PRKAA1 antibody.
Limitation:
This PRKAA1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13131