RhoF Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6337
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6337-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the RhoF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human HeLa, 2) human A549, 3) rat brain and 4) mouse brain lysate with RhoF antibody. Predicted molecular weight ~23 kDa.
2 / 2
Flow cytometry testing of human A549 cells with RhoF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RhoF antibody.
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Western blot testing of 1) human HeLa, 2) human A549, 3) rat brain and 4) mouse brain lysate with RhoF antibody. Predicted molecular weight ~23 kDa.
Flow cytometry testing of human A549 cells with RhoF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RhoF antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the RhoF antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Rho, the Ras-related small GTPase, is responsible for the regulation of actin-based cytoskeletal structures including stress fibers, focal adhesions and the contractile ring apparatus. Rho proteins function as molecular switches that are able to turn cytokinesis on and off. Although little is known about signaling downstream of Rho, a host of putative Rho effector proteins have been described. Rho F (ras homolog gene family, member F), also known as RIF or ARHF, is a 211 amino acid membrane protein that localizes to the cytoplasmic side of the plasma membrane. Belonging to the small GTPase superfamily and the Rho family, Rho F functions cooperatively with Cdc42 and Rac to generate additional cytoskeletal structures, such as increasing variation of actin-based morphology. Rho F exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 12q24.31.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Y39-K204) was used as the immunogen for the RhoF antibody.
Limitation:
This RhoF antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9HBH0