RIO Kinase 1 Antibody / RIOK1 / RIO1

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NSJ Bioreagents
Product Code: NSJ-RQ5835
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5835-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the RIO Kinase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human HL-60 cells with RIO Kinase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RIO Kinase 1 antibody.
2 / 2
Western blot testing of 1) K562, 2) HEK293, 3) HepG2, 4) PC-3, 5) rat testis, 6) mouse testis and 7) mouse SP2/0 lysate with RIO Kinase 1 antibody. Predicted molecular weight ~65 kDa.
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Flow cytometry testing of human HL-60 cells with RIO Kinase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RIO Kinase 1 antibody.
Western blot testing of 1) K562, 2) HEK293, 3) HepG2, 4) PC-3, 5) rat testis, 6) mouse testis and 7) mouse SP2/0 lysate with RIO Kinase 1 antibody. Predicted molecular weight ~65 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the RIO Kinase 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Serine/threonine-protein kinase RIO1 is an enzyme that in humans is encode by the RIOK1 gene. The protein encoded by this gene competes with pICln for inclusion in the protein arginine methyltransferase 5 complex. This complex targets substrates for dimethylation. The encoded protein is essential for the last steps in the maturation of 40S subunits.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-K222) was used as the immunogen for the RIO Kinase 1 antibody.
Limitation:
This RIO Kinase 1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9BRS2