SRD5A1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6397
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6397-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the SRD5A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human PC-3 cells with SRD5A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SRD5A1 antibody.
2 / 3
Flow cytometry testing of rat RH35 cells with SRD5A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SRD5A1 antibody.
3 / 3
Western blot testing of 1) rat liver, 2) rat kidney, 3) rat testis, 4) mouse liver, 5) mouse kidney and 6) mouse testis tissue lysate with SRD5A1 antibody. Predicted molecular weight ~29 kDa.
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Flow cytometry testing of human PC-3 cells with SRD5A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SRD5A1 antibody.
Flow cytometry testing of rat RH35 cells with SRD5A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SRD5A1 antibody.
Western blot testing of 1) rat liver, 2) rat kidney, 3) rat testis, 4) mouse liver, 5) mouse kidney and 6) mouse testis tissue lysate with SRD5A1 antibody. Predicted molecular weight ~29 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SRD5A1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. [UniProt]
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Y38-R251) was used as the immunogen for the SRD5A1 antibody.
Limitation:
This SRD5A1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P18405