TSG101 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5849
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5849-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the TSG101 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Western blot testing of rat 1) brain, 2) testis, 3) liver, 4) spleen and mouse 5) brain, 6) testis, 7) liver and 8) spleen lysate with TSG101 antibody. Predicted molecular weight ~45 kDa.
2 / 3
Western blot testing of human 1) placenta, 2) COLO-320, 3) SW620, 4) Caco-2, 5) HEK293, 6) K562, 7) HepG2 and 8) ThP-1 lysate with TSG101 antibody. Predicted molecular weight ~45 kDa.
3 / 3
Flow cytometry testing of human SiHa cells with TSG101 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TSG101 antibody.
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Western blot testing of rat 1) brain, 2) testis, 3) liver, 4) spleen and mouse 5) brain, 6) testis, 7) liver and 8) spleen lysate with TSG101 antibody. Predicted molecular weight ~45 kDa.
Western blot testing of human 1) placenta, 2) COLO-320, 3) SW620, 4) Caco-2, 5) HEK293, 6) K562, 7) HepG2 and 8) ThP-1 lysate with TSG101 antibody. Predicted molecular weight ~45 kDa.
Flow cytometry testing of human SiHa cells with TSG101 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TSG101 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TSG101 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
TSG101, known as Tumor susceptibility gene 101, is mapped to 11p15. The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. And the protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E223-K257) was used as the immunogen for the TSG101 antibody.
Limitation:
This TSG101 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q99816