N-WASP Antibody / WASL

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NSJ Bioreagents
Product Code: NSJ-RQ6160
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6160-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the WASL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human HeLa cells with WASL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WASL antibody.
2 / 2
Western blot testing of 1) human SH-SY5Y, 2) human U-87 MG, 3) human HeLa, 4) human Caco-2, 5) rat brain, 6) rat lung, 7) rat liver, 8) rat C6, 9) mouse brain, 10) mouse lung, 11) mouse liver and 12) mouse Neuro-2a lysate with WASL antibody. Predicted molecular weight ~65 kDa.
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Flow cytometry testing of human HeLa cells with WASL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WASL antibody.
Western blot testing of 1) human SH-SY5Y, 2) human U-87 MG, 3) human HeLa, 4) human Caco-2, 5) rat brain, 6) rat lung, 7) rat liver, 8) rat C6, 9) mouse brain, 10) mouse lung, 11) mouse liver and 12) mouse Neuro-2a lysate with WASL antibody. Predicted molecular weight ~65 kDa.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the WASL antibody should be determined by the researcher.
Description:
Neural Wiskott-Aldrich syndrome protein is a protein that in humans is encoded by the WASL gene. This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids Q5-H211) was used as the immunogen for the WASL antibody.
Limitation:
This WASL antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O00401