ETV5 Antibody / ETS Translocation Variant 5

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NSJ Bioreagents
Product Code: NSJ-RQ5812
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5812-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the ETS Variant 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human MCF7 cells with ETS Variant 5 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) A549, 2) SW620, 3) HepG2, 4) rat C6, 5) mouse Neuro-2a and 6) mouse HEPA1-6 lysate with ETS Variant 5 antibody. Expected molecular weight: 58-70 kDa, doublet may be observed.
3 / 3
Flow cytometry testing of human HL-60 cells with ETS Variant 5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ETS Variant 5 antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with ETS Variant 5 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) A549, 2) SW620, 3) HepG2, 4) rat C6, 5) mouse Neuro-2a and 6) mouse HEPA1-6 lysate with ETS Variant 5 antibody. Expected molecular weight: 58-70 kDa, doublet may be observed.
Flow cytometry testing of human HL-60 cells with ETS Variant 5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ETS Variant 5 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ETS Variant 5 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Ets variant 5 (ETV5) (also named ERM transcription factor) is a transcription factor that in humans is encoded by the ETV5 gene. It is generated in Sertoli cells, which are found in the testes and play a crucial role in spermatogenesis. Its ortholog has been linked to both obesity and bipolar disorder.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids D72-Q246) was used as the immunogen for the ETS Variant 5 antibody.
Limitation:
This ETS Variant 5 antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P41161