KIBRA Antibody / WWC1

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NSJ Bioreagents
Product Code: NSJ-RQ5851
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5851-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the KIBRA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human A431 cells with KIBRA antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) MCF7, 2) MDA-MB-453, 3) HEK293, 4) rat brain and 5) mouse brain lysate with KIBRA antibody. Predicted molecular weight ~125 kDa.
3 / 3
Flow cytometry testing of human A431 cells with KIBRA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KIBRA antibody.
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Immunofluorescent staining of FFPE human A431 cells with KIBRA antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) MCF7, 2) MDA-MB-453, 3) HEK293, 4) rat brain and 5) mouse brain lysate with KIBRA antibody. Predicted molecular weight ~125 kDa.
Flow cytometry testing of human A431 cells with KIBRA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KIBRA antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the KIBRA antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Protein KIBRA also known as 'kidney and brain expressed protein' or WW domain-containing protein 1 (WWC1) is a protein that in humans is encoded by the WWC1 gene. The protein encoded by this gene is a cytoplasmic phosphoprotein that interacts with PRKC-zeta and dynein light chain-1. Alleles of this gene have been found that enhance memory in some individuals. Three transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids D990-Q1080) was used as the immunogen for the KIBRA antibody.
Limitation:
This KIBRA antibody is available for research use only.
Localization:
Cytoplasmic, nuclear, plasma membrane
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q8IX03