PFAS Antibody / Phosphoribosylformylglycinamidine synthase

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NSJ Bioreagents
Product Code: NSJ-RQ6391
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6391-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the PFAS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human HeLa cells with PFAS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) placenta, 2) A549, 3) HEK293, 4) HeLa, 5) K562, 6) U937, 7) HepG2 and rat 8) liver, 9) brain, 10) testis and mouse 11) liver, 12) brain and 13) testis tissue lysate with PFAS antibody. Predicted molecular weight ~145 kDa.
3 / 3
Flow cytometry testing of human HepG2 cells with PFAS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PFAS antibody.
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Immunofluorescent staining of FFPE human HeLa cells with PFAS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) placenta, 2) A549, 3) HEK293, 4) HeLa, 5) K562, 6) U937, 7) HepG2 and rat 8) liver, 9) brain, 10) testis and mouse 11) liver, 12) brain and 13) testis tissue lysate with PFAS antibody. Predicted molecular weight ~145 kDa.
Flow cytometry testing of human HepG2 cells with PFAS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PFAS antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PFAS antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. The enzyme encoded by this gene catalyzes the fourth step of IMP biosynthesis.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R330-S569) was used as the immunogen for the PFAS antibody.
Limitation:
This PFAS antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O15067