PTF1A Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ6003
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6003-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the PTF1A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human A431 cells with PTF1A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) rat pancreas, 2) mouse pancreas, 3) human PANC-1, 4) human PANC-1, 5) human U-2 OS and 6) mouse SP2/0 lysate with PTF1A antibody. Predicted molecular weight: ~35 kDa, routinely observed at ~48 kDa. (Ref 1)
3 / 3
Flow cytometry testing of human A431 cells with PTF1A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PTF1A antibody.
❮❯

Immunofluorescent staining of FFPE human A431 cells with PTF1A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) rat pancreas, 2) mouse pancreas, 3) human PANC-1, 4) human PANC-1, 5) human U-2 OS and 6) mouse SP2/0 lysate with PTF1A antibody. Predicted molecular weight: ~35 kDa, routinely observed at ~48 kDa. (Ref 1)
Flow cytometry testing of human A431 cells with PTF1A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PTF1A antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PTF1A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Pancreas transcription factor 1 subunit alpha is a protein that in humans is encoded by the PTF1A gene. It is mapped to 10p12.2. This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-Q292) was used as the immunogen for the PTF1A antibody.
Limitation:
This PTF1A antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q7RTS3