SOX18 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6340
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6340-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the SOX18 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human HepG2 cells with SOX18 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human Jurkat, 2) human COLO-320, 3) human HeLa, 4) human HepG2, 5) monkey COS-7, 6) rat heart, 7) rat lung, 8) rat liver, 9) mouse lung and 10) mouse liver lysate with SOX18 antibody. Predicted molecular weight ~41 kDa.
3 / 3
Flow cytometry testing of human HepG2 cells with SOX18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SOX18 antibody.
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Immunofluorescent staining of FFPE human HepG2 cells with SOX18 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human Jurkat, 2) human COLO-320, 3) human HeLa, 4) human HepG2, 5) monkey COS-7, 6) rat heart, 7) rat lung, 8) rat liver, 9) mouse lung and 10) mouse liver lysate with SOX18 antibody. Predicted molecular weight ~41 kDa.
Flow cytometry testing of human HepG2 cells with SOX18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SOX18 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cellsDirect ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SOX18 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Transcription factor SOX-18 is a protein that in humans is encoded by the SOX18 gene. This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. This protein plays a role in hair, blood vessel, and lymphatic vessel development. Mutations in this gene have been associated with recessive and dominant forms of hypotrichosis-lymphedema-telangiectasia.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E63-E362) was used as the immunogen for the SOX18 antibody.
Limitation:
This SOX18 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P35713