WAC Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5927
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5927-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the WAC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human A431 cells with WAC antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) A431, 2) ThP-1, 3) Raji, 4) K562, 5) U-2 OS, 6) SK-O-V3, 7) rat brain, 8) rat PC-12, 9) mouse thymus and 10) mouse SP2/0 lysate with WAC antibody. Expected molecular weight: 70-80 kDa.
3 / 3
Flow cytometry testing of human HepG2 cells with WAC antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WAC antibody.
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Immunofluorescent staining of FFPE human A431 cells with WAC antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) A431, 2) ThP-1, 3) Raji, 4) K562, 5) U-2 OS, 6) SK-O-V3, 7) rat brain, 8) rat PC-12, 9) mouse thymus and 10) mouse SP2/0 lysate with WAC antibody. Expected molecular weight: 70-80 kDa.
Flow cytometry testing of human HepG2 cells with WAC antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WAC antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the WAC antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
The protein encoded by this gene contains a WW domain, which is a protein module found in a wide range of signaling proteins. This domain mediates protein-protein interactions and binds proteins containing short linear peptide motifs that are proline-rich or contain at least one proline. This gene product shares 94% sequence identity with the WAC protein in mouse, however, its exact function is not known. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Q459-V647) was used as the immunogen for the WAC antibody.
Limitation:
This WAC antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9BTA9