AFAP1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6241
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6241-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the AFAP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human lung cancer with AFAP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human prostate cancer with AFAP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Western blot testing of 1) human Hela, 2) human SH-SY5Y, 3) human A431, 4) human U-87 MG, 5) rat brain, 6) rat lung, 7) rat stomach, 8) mouse brain, 9) mouse lung and 10) mouse stomach lysate with AFAP1 antibody. Predicted molecular weight ~120 kDa.
4 / 5
Flow cytometry testing of human U-2 OS cells with AFAP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AFAP1 antibody.
5 / 5
Flow cytometry testing of human A549 cells with AFAP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AFAP1 antibody.
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IHC staining of FFPE human lung cancer with AFAP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer with AFAP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human Hela, 2) human SH-SY5Y, 3) human A431, 4) human U-87 MG, 5) rat brain, 6) rat lung, 7) rat stomach, 8) mouse brain, 9) mouse lung and 10) mouse stomach lysate with AFAP1 antibody. Predicted molecular weight ~120 kDa.
Flow cytometry testing of human U-2 OS cells with AFAP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AFAP1 antibody.
Flow cytometry testing of human A549 cells with AFAP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AFAP1 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the AFAP1 antibody should be determined by the researcher.
Description:
Actin filament-associated protein 1 is a protein that in humans is encoded by the AFAP1 gene. The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids A498-T730) was used as the immunogen for the AFAP1 antibody.
Limitation:
This AFAP1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q8N556