Coronin 1a Antibody / TACO / CORO1A

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NSJ Bioreagents
Product Code: NSJ-RQ6415
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6415-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Coronin 1a antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 11
IHC staining of FFPE human gallbladder adenocarcinoma tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 11
IHC staining of FFPE human renal clear cell carcinoma tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 11
IHC staining of FFPE human lung cancer tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 11
IHC staining of FFPE human liver cancer tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 11
IHC staining of FFPE human gastric cancer tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE human colonic adenocarcinoma tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
IHC staining of FFPE human ovarian cancer tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 11
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 11
Western blot testing of 1) human Jurkat, 2) rat spleen, 3) rat thymus, 4) mouse spleen and 5) mouse thymus tissue lysate with Coronin 1a antibody. Predicted molecular weight ~51 kDa.
10 / 11
Flow cytometry testing of human U937 cells with Coronin 1a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Coronin 1a antibody.
11 / 11
Flow cytometry testing of human ThP-1 cells with Coronin 1a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Coronin 1a antibody.
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IHC staining of FFPE human gallbladder adenocarcinoma tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal clear cell carcinoma tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric cancer tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colonic adenocarcinoma tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with Coronin 1a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human Jurkat, 2) rat spleen, 3) rat thymus, 4) mouse spleen and 5) mouse thymus tissue lysate with Coronin 1a antibody. Predicted molecular weight ~51 kDa.
Flow cytometry testing of human U937 cells with Coronin 1a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Coronin 1a antibody.
Flow cytometry testing of human ThP-1 cells with Coronin 1a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Coronin 1a antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Coronin 1a antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Coronin-1A, also called TACO or Tryptophan aspartate-containing coat protein, is a protein that in humans is encoded by the CORO1A gene. This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E173-K461) was used as the immunogen for the Coronin 1a antibody.
Limitation:
This Coronin 1a antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P31146