Diacylglycerol kinase zeta Antibody / DGKZ

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NSJ Bioreagents
Product Code: NSJ-RQ6125
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6125-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Diacylglycerol kinase zeta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human breast cancer with Diacylglycerol kinase zeta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human liver cancer with Diacylglycerol kinase zeta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human tonsil with Diacylglycerol kinase zeta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE mouse brain tissue with Diacylglycerol kinase zeta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of 1) human T-47D, 2) human ThP-1, 3) rat brain and 4) mouse brain lysate with Diacylglycerol kinase zeta antibody. Predicted molecular weight ~124 kDa.
6 / 6
Flow cytometry testing of human HL60 cells with Diacylglycerol kinase zeta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Diacylglycerol kinase zeta antibody.
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IHC staining of FFPE human breast cancer with Diacylglycerol kinase zeta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with Diacylglycerol kinase zeta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with Diacylglycerol kinase zeta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with Diacylglycerol kinase zeta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human T-47D, 2) human ThP-1, 3) rat brain and 4) mouse brain lysate with Diacylglycerol kinase zeta antibody. Predicted molecular weight ~124 kDa.
Flow cytometry testing of human HL60 cells with Diacylglycerol kinase zeta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Diacylglycerol kinase zeta antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Diacylglycerol kinase zeta antibody should be determined by the researcher.
Description:
Diacylglycerol kinase zeta is an enzyme that in humans is encoded by the DGKZ gene. The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids E664-V928) was used as the immunogen for the Diacylglycerol kinase zeta antibody.
Limitation:
This Diacylglycerol kinase zeta antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13574