GFI1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5979
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5979-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the GFI1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE human rectal cancer with GFI1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of 1) rat spleen, 2) mouse thymus, 3) mouse RAW264.7, 4) mouse ANA-1, 5) human ThP-1 and 6) human Raji lysate with GFI1 antibody. Expected molecular weight: 47-55 kDa.
3 / 3
Flow cytometry testing of human ThP-1 cells with GFI1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GFI1 antibody.
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IHC staining of FFPE human rectal cancer with GFI1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat spleen, 2) mouse thymus, 3) mouse RAW264.7, 4) mouse ANA-1, 5) human ThP-1 and 6) human Raji lysate with GFI1 antibody. Expected molecular weight: 47-55 kDa.
Flow cytometry testing of human ThP-1 cells with GFI1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GFI1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the GFI1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Zinc finger protein Gfi-1 is a transcriptional repressor that in humans is encoded by the GFI1 gene. It is mapped to 1p22.1. This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-K422) was used as the immunogen for the GFI1 antibody.
Limitation:
This GFI1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q99684