Glutamate-oxaloacetate transaminase 2 Antibody / GOT2 / FABP-1

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NSJ Bioreagents
Product Code: NSJ-RQ6424
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6424-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Glutamate-oxaloacetate transaminase 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human ovarian cancer tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 14
IHC staining of FFPE human gastric cancer tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 14
IHC staining of FFPE human lung cancer tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 14
IHC staining of FFPE human renal carcinoma tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 14
IHC staining of FFPE human breast cancer tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 14
IHC staining of FFPE human gallbladder adenocarcinoma tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 14
IHC staining of FFPE human adrenal cortical adenocarcinoma tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 14
IHC staining of FFPE mouse brain tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 14
IHC staining of FFPE mouse lymph node tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 14
IHC staining of FFPE rat brain tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
11 / 14
IHC staining of FFPE rat lymph node tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
12 / 14
Immunofluorescent staining of FFPE human T-47D cells with Glutamate-oxaloacetate transaminase 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
13 / 14
Western blot testing of human 1) HeLa, 2) PC-3, 3) Raji, 4) Jurkat, 5) A549, 6) HepG2, 7) Caco-2, 8) U937, 9) rat brain, 10) rat liver, 11) rat kidney, 12) rat ovary, 13) mouse brain, 14) mouse liver, 15) mouse kidney and 16) mouse ovary tissue lysate with Glutamate-oxaloacetate transaminase 2 antibody. Predicted molecular weight ~47 kDa.
14 / 14
Flow cytometry testing of human HepG2 cells with Glutamate-oxaloacetate transaminase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Glutamate-oxaloacetate transaminase 2 antibody.
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IHC staining of FFPE human ovarian cancer tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric cancer tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal carcinoma tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gallbladder adenocarcinoma tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human adrenal cortical adenocarcinoma tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse lymph node tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat lymph node tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human T-47D cells with Glutamate-oxaloacetate transaminase 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) PC-3, 3) Raji, 4) Jurkat, 5) A549, 6) HepG2, 7) Caco-2, 8) U937, 9) rat brain, 10) rat liver, 11) rat kidney, 12) rat ovary, 13) mouse brain, 14) mouse liver, 15) mouse kidney and 16) mouse ovary tissue lysate with Glutamate-oxaloacetate transaminase 2 antibody. Predicted molecular weight ~47 kDa.
Flow cytometry testing of human HepG2 cells with Glutamate-oxaloacetate transaminase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Glutamate-oxaloacetate transaminase 2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Glutamate-oxaloacetate transaminase 2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Aspartate aminotransferase, mitochondrial is an enzyme that in humans is encoded by the GOT2 gene. Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids H35-K430) was used as the immunogen for the Glutamate-oxaloacetate transaminase 2 antibody.
Limitation:
This Glutamate-oxaloacetate transaminase 2 antibody is available for research use only.
Localization:
Cytoplasmic, cell membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P00505