Glycine Decarboxylase Antibody / GLDC

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NSJ Bioreagents
Product Code: NSJ-RQ6128
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6128-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Glycine decarboxylase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human bladder cancer with Glycine decarboxylase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human testicular cancer with Glycine decarboxylase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human placenta with Glycine decarboxylase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE mouse liver with Glycine decarboxylase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat liver with Glycine decarboxylase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 7
Western blot testing of human 1) HepG2, 2) placenta, 3) HEK293, 4) T-47D, 5) A549, 6) rat liver, 7) rat kidney, 8) mouse liver and 9) mouse kidney lysate with Glycine decarboxylase antibody. Predicted molecular weight ~113 kDa.
7 / 7
Flow cytometry testing of human Caco-2 cells with Glycine decarboxylase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Glycine decarboxylase antibody.
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IHC staining of FFPE human bladder cancer with Glycine decarboxylase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human testicular cancer with Glycine decarboxylase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placenta with Glycine decarboxylase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse liver with Glycine decarboxylase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver with Glycine decarboxylase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HepG2, 2) placenta, 3) HEK293, 4) T-47D, 5) A549, 6) rat liver, 7) rat kidney, 8) mouse liver and 9) mouse kidney lysate with Glycine decarboxylase antibody. Predicted molecular weight ~113 kDa.
Flow cytometry testing of human Caco-2 cells with Glycine decarboxylase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Glycine decarboxylase antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Glycine decarboxylase antibody should be determined by the researcher.
Description:
Glycine decarboxylase also known as glycine cleavage system P protein or glycine dehydrogenase is an enzyme that in humans is encoded by the GLDC gene. Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids K574-S1020) was used as the immunogen for the Glycine decarboxylase antibody.
Limitation:
This Glycine decarboxylase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P23378