K-Cadherin Antibody / CDH6

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NSJ Bioreagents
Product Code: NSJ-RQ6246
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6246-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the K-Cadherin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE human ovarian cancer with K-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of human 1) HeLa, 2) A549, 3) U-2 OS, 4) U-87 MG, 5) A431, 6) rat lung and 7) mouse lung lysate with K-Cadherin antibody. Predicted molecular weight ~88 kDa.
3 / 3
Flow cytometry testing of human PC-3 cells with K-Cadherin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= K-Cadherin antibody.
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IHC staining of FFPE human ovarian cancer with K-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) A549, 3) U-2 OS, 4) U-87 MG, 5) A431, 6) rat lung and 7) mouse lung lysate with K-Cadherin antibody. Predicted molecular weight ~88 kDa.
Flow cytometry testing of human PC-3 cells with K-Cadherin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= K-Cadherin antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the K-Cadherin antibody should be determined by the researcher.
Description:
Cadherin-6 is a protein that in humans is encoded by the CDH6 gene. This gene encodes a member of the cadherin superfamily. Cadherins are membrane glycoproteins that mediate homophilic cell-cell adhesion and play critical roles in cell differentiation and morphogenesis. The encoded protein is a type II cadherin and may play a role in kidney development as well as endometrium and placenta formation. Decreased expression of this gene may be associated with tumor growth and metastasis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids N153-H424) was used as the immunogen for the K-Cadherin antibody.
Limitation:
This K-Cadherin antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P55285