KCNJ8 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6193
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6193-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the KCNJ8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE human pancreatic cancer with KCNJ8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of human 1) Caco-2, 2) SH-SY5Y, 3) HEK293, 4) K562, 5) A549, 6) rat RH35, 7) mouse kidney and 8) mouse HEPA1-6 lysate with KCNJ8 antibody. Predicted molecular weight ~48 kDa.
3 / 3
Flow cytometry testing of human U-2 OS cells with KCNJ8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KCNJ8 antibody.
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IHC staining of FFPE human pancreatic cancer with KCNJ8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) Caco-2, 2) SH-SY5Y, 3) HEK293, 4) K562, 5) A549, 6) rat RH35, 7) mouse kidney and 8) mouse HEPA1-6 lysate with KCNJ8 antibody. Predicted molecular weight ~48 kDa.
Flow cytometry testing of human U-2 OS cells with KCNJ8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KCNJ8 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the KCNJ8 antibody should be determined by the researcher.
Description:
Potassium inwardly-rectifying channel, subfamily J, member 8, also known as KCNJ8, is a human gene encoding the Kir6.1 protein. Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. Defects in this gene may be a cause of J-wave syndromes and sudden infant death syndrome (SIDS).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids M1-S424) was used as the immunogen for the KCNJ8 antibody.
Limitation:
This KCNJ8 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q15842