MCU Antibody / Calcium uniporter protein, mitochondrial

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NSJ Bioreagents
Product Code: NSJ-RQ5827
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5827-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MCU antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human liver cancer with MCU antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human bladder cancer with MCU antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE mouse lung with MCU antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE rat lung with MCU antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of human 1) HeLa, 2) A549, 3) PANC-1, 4) monkey COS-7, 5) HL-60, 6) HepG2, 7) Jurkat and rat 8) brain, 9) kidney, 10) heart, 11) PC-12 and mouse 12) brain, 13) kidney, 14) heart and 15) NIH 3T3 lysate with MCU antibody. Predicted molecular weight ~34 kDa.
6 / 6
Flow cytometry testing of human HeLa cells with MCU antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCU antibody.
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IHC staining of FFPE human liver cancer with MCU antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder cancer with MCU antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse lung with MCU antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat lung with MCU antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) A549, 3) PANC-1, 4) monkey COS-7, 5) HL-60, 6) HepG2, 7) Jurkat and rat 8) brain, 9) kidney, 10) heart, 11) PC-12 and mouse 12) brain, 13) kidney, 14) heart and 15) NIH 3T3 lysate with MCU antibody. Predicted molecular weight ~34 kDa.
Flow cytometry testing of human HeLa cells with MCU antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCU antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the MCU antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
The mitochondrial calcium uniporter (MCU) is a transmembrane protein that allows the passage of calcium ions from a cell's cytosol into mitochondria. Its activity is regulated by MICU1 and MICU2, which together with the MCU make up the mitochondrial calcium uniporter complex. This gene encodes a calcium transporter that localizes to the mitochondrial inner membrane. The encoded protein interacts with mitochondrial calcium uptake 1. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids V51-D351) was used as the immunogen for the MCU antibody.
Limitation:
This MCU antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q8NE86